Obtaining high stable yields of agricultural crops is the main objective of farmers. Increasing from year to year in Russia the cost of fertilizers, plant protection products and energy is forcing rural producers to find new low cost ways of increasing the crop production. One of the most promising areas of modern technology of crop production is the use of biological agents and stimulators of plant growth. Stimulators of growth activate the immune system of plants, allows to “smooth out” the limiting factors of obtaining potential yield – increase resistance to drought or excess moisture, high or low ambient temperatures, as well as to speed up or slow down the maturation of plants, increase the number of ovaries, contribute to the redistribution of nutrients in economically important plant organs. Achievement which is rarely provided by traditional technologies. This study was conducted on the influence of growth stimulants Epin, Immunocytophit and Zircon on yield and grain quality of winter rye. The experiments were conducted in 2014 and 2015 in the level of crop rotation on the experimental field of the Mari state University. It was revealed that the treatment of crops of winter rye growth stimulants Epin and Zircon significantly increases grain yield. In this case the higher yield of winter rye was obtained in variant with the application of Epin – 2,93 t/ha. In the processing of crops Zircon grain yield was 3,8 % lower. Studies have also shown that the use of growth stimulants improves the product quality of winter rye. Thus, the protein content in grain, depending on the variant, it was 0,28–0,88 % more the control. Higher protein content was observed when using Epin was 12,61 %. The carbohydrate-amylase complex of grain of a winter rye of the studied variants corresponded the technological requirements.