The most perspective lines of soy bean were studied concerning separation of samples on the active symbiotic activity of plants. It’s found out that both genotypic aspects and abiotic factors influence on active symbiotic and photosynthetic activity of soy bean sowing. It’s stated that the best conditions of active legumes nodule bacteria symbiosis are made with optimal supply of moisture in critical phase of growth and development of soy bean. The study highlighted the perspective samples in biological fixing of air nitrogen for the future research in selective soy bean process.