Introduction. In addition to saprophytic microbes, infectious agents are often detected in the soil. They can contaminate the soil with a variety of organic waste. Sanitary-indicative microbes are able to persist and/or multiply for a rather long period. Screening of microbial isolates − antagonists of sanitary-indicative microbes is promising. Their use would allow accelerating the inactivation of pathogenic microorganisms in the soil. The purpose of the research is to search for antagonists in relation to sanitary-indicative microbes. Materials and methods. Isolates of microorganisms isolated from soil samples from various regions of the Republic of Tatarstan served as the material for the study. Antagonism was detected by the “stroke” and “dish” methods by inoculation on dense and liquid special nutrient media. The following microorganisms were used as test strains: L. monocytogenes, Staph. aureus, C. albicans, Salm. typhimurium, E. coli. Research results, discussion. Isolates of micromycetes, actinomycetes and bacteria were isolated and obtained. Of the bacteria (thirty-two isolates in total), antagonism was shown: to Sal. typhimurium − six isolates, to E. coli − four isolates, to L. monocitogenes − five isolates, to C. albicans − two isolates, to St. aureus − twelve isolates. Actinomycetes (ten isolates in total) showed antagonism: to S. typhimurium − two isolates, to St. aureus − seven isolates, to L. monocitogenes − two isolates, to C. albicans − one isolate, to E. coli − eight isolates. Micromycetes (sixty-one isolates in total) showed antagonism against St. aureus − five isolates, E. coli − three isolates. Bacillus isolates had a more pronounced antagonistic effect. Conclusion. Isolate of actinomycete Streptomyces AS31/2, isolates of Bacillus spp. under codes BSB3, BSB4, BSB5 and soil micromycete Trichoderma TR2 were selected as promising for subsequent experiments.