The article aims to review various cell culture or in vitro co-culture models that can be used to assess mycotoxin exposure, toxicity and risk reduction, and their suitability and limitations for assessing the safety of animal foods and by-products. Mycotoxins are a worldwide problem because of their potential to contaminate agricul-tural commodities and pose a health risk to both humans and animals. Since mycotoxins are usually present in combination, it is important not only to understand their mechanisms of toxic action, many of which are un-known, but also to understand how they interact with each other to affect humans and animals exposed to them. In vitro cell culture models of the intestinal barrier were used to simulate oral exposure to mycotoxins. These intestinal models are usually based on a monolayer epithelial cell culture system, sometimes grown on membrane inserts, to better mimic the intestinal barrier for assessing intestinal mycotoxin transport. A number of different mitigation approaches have been developed and applied to help reduce the adverse effects of my-cotoxins on animals. With this in mind, there is a constant need to develop new, more effective mycotoxin ad-sorbents and to evaluate their effectiveness.In vitro cell culture can help you better understand what is actually happening at the gut level. In vitro cell culture systems (monoculture or co-culture systems) can be a useful and effective approach to start exploring the complex problems of organ and species-specific mycotoxin toxicity at the cellular and molecular levels, such as interactions between different mycotoxins, the comparative toxicity of mycotoxins and their metabolites. Cell culture systems may be suitable methods for studying the biotransfor-mation of mycotoxins in animal cells and protective agents.