Blood groups are used in animal breeding to improve the efficiency of selection and breeding work. One of the areas of application of blood groups is to identify markers of high productivity, reproductive qualities, and so on. This article presents research results of the immunogenetic blood test by bulls of black-pied and holstein breeds from JSC “Maiyskoe”. The study calculated the frequency of antigens in blood group in the test herd bulls. The research has shown that the evaluated bulls-producers have only antigens of seven systems of blood groups of the eight. There were no antigens of EAM blood group system. Studies have shown that the erythrocyte antigens of blood groups of cattle mark the indicators of sperm productivity and its fertility. Identification and use of such markers will allow for the selection of bulls with a view to complete the necessary herds genotypes. The influence of B-system antigens on reproductive qualities of stud bulls in JSC “Maiyskoe” was found. So, antigens А2', U, F may serve as a marker of high reproductive capacity.